Prevalence of Overnutrition using Body Mass Index in Comparison with Bioelectrical Impedance Analysis among Children and Adolescents in Benin, Nigeria

Aim: To compare the prevalence of overweight and obesity among school children in Egor Local Government Area (LGA) using body mass index (BMI) and bioelectricial impedance analysis (BIA) and determine the ability of body mass index (BMI) to predict body fat mass as measured by bioelectricial impedance analysis (BIA). Study Design: This was a cross sectional study conducted in Egor Local Government Area of Edo State of Nigeria with1067 children aged 6-18 years recruited from private and public schools. Body mass index was calculated as weight in kilograms divided by the square of height in meters while Body fat was estimated by BIA using a Tanita scale. Place and Duration of Study: The study took place in selected private and public primary/secondary schools in Egor LGA of Edo State. The study was conducted over a three-month period from October to December 2017. Methodology: Using multi-stage sampling technique,1067 children aged 6-18 years were recruited from private and public schools in Egor Local Government Area in Benin City, Nigeria. Body fat was estimated by BIA using a Tanita scale, whereas BMI was calculated as weight in kilograms divided by the square of height in meters. Data was analyzed using SPSS version 21. Results: A total of 1067 pupils consisting of 538 (50.4%) males and 529 (49.6%) females were recruited. The overall prevalence of overnutrition by BMI, 13.4% (overweight 9.6% and obesity 3.8%) was comparable to that by BIA which was 12.4% (overweight 5.4% and obesity 6.9%). BMI showed a strong positive correlation with fat mass, and fat mass index especially in females (0.917, 0.907, p < 0.000). Conclusion: The prevalence of overnutrition using BMI and BIA was comparable. The prevalence of obesity is however underestimated with BMI.

Cambios en la composición corporal y ángulo de fase durante la pretemporada en jugadores de fútbol profesional / Changes in body composition and phase angle during pre-season in professional soccer players

RESUMEN: El análisis y el control de la composición corporal son claves en el fútbol por su implicación en el rendimiento. El objetivo de este estudio fue identificar, en jugadores de futbol profesional, el ángulo de fase (PhA) de miembros inferiores con los posibles patrones de mejora del componente magro en los mismos medido por DEXA. Al mismo tiempo, se estudió mediante BIA la evolución de parámetros hídricos de los jugadores, sometidos a un entrenamiento intenso y a un control nutricional de su alimentación y suplementación. Se evaluaron a 18 jugadores (26,28 ± 5,1 años; 85,09 ± 9,16 kg; 185,5 ± 4,32 cm) de un equipo de la primera división de fútbol italiana. Se llevó a cabo el estudio durante cuatro semanas, entre el 11 de julio (pre-test) y el 11 de agosto de 2021 (post-test). La intervención se llevó a cabo en su totalidad en pretemporada, con un confinamiento en modalidad de aislamiento o burbuja total por prevención al contagio del COVID-19, dónde se monitorizaba en un alto porcentaje la vida del jugador, incluyendo factores como la alimentación, el entrenamiento y el descanso. La suplementación estuvo basada en antioxidantes, multivitamínicos, minerales y ácidos grasos poliinsaturados y dos productos en polvo (uno a base de creatina, glutamina y leucina, y otro, a base de aminoácidos esenciales). Los jugadores presentaron una mejora del PhA y del componente magro en los miembros inferiores, con una moderada correlación (r = 0,6). Con respecto al agua intracelular y extracelular, señalar que, a pesar de la alta intensidad del ejercicio durante cuatro semanas, éstas se han mantenido constantes sin presentar variaciones significativas durante el periodo de estudio, lo que indica que no se ha producido un proceso de deshidratación del jugador.

SUMMARY: The analysis and control of body composition is essential in soccer due to its implication in performance. The aim of this study was to identify, in professional soccer players, the phase angle (PhA) of the lower limbs with possible patterns of improvement of the lean component measured by DEXA. At the same time, the evolution of hydric parameters of the players, subjected to intense training and nutritional control of their diet and supplementation, was studied by means of BIA. Eighteen players (26.28 ± 5.1 years; 85.09 ± 9.16 kg; 185.5 ± 4.32 cm) from an Italian first division football team were evaluated. The study was conducted for four weeks, between 11th July (pre- test) and 11th August of 2021 (post-test). The intervention was carried out entirely in pre-season, with confinement in isolation or total bubble mode for prevention of COVID-19 infection, where a high percentage of the player's life was monitored, including factors such as diet, training and rest. Supplementation was based on antioxidants, multivitamins, minerals and polyunsaturated fatty acids and two powdered products (one based on creatine, glutamine and leucine, and the other one, on essential amino acids). The players showed an improvement in PhA and in the lean component in the lower limbs, with a moderate correlation (r = 0.6). With regard to intracellular and extracellular water, it should be noted that, despite the high intensity of exercise during four weeks, these have remained constant without significant variations during the study period, indicating that there has not been a process of dehydration of the player.

Características clínicas y quirúrgicas de pacientes sometidos a cirugía de válvula mitral en una ins titución de salud de Barranquilla, Colombia / Clinic al and surgic al characteristics of patients undergoing mitral valve surgery at a health ins titution in Barranquilla, Colombia

Introducción: la incidencia de las anomalías valvulares cardiacas ha aumentado entre otras razones por los avances en métodos diagnósticos y conocimiento de predecesores etiológicos como desenca­ denantes de la condición clínica, que inicia la probabilidad diagnostica y seguimiento temprano en pa­ cientes en estadios iniciales de la enfermedad, disminuyendo de esta manera el impacto económico por aumento de la morbimortalidad. Objetivo: describir las características clínicas y quirúrgicas de pa­ cientes sometidos a cirugía de válvula mitral en una institución prestadora de servicios de salud de re­ ferencia para el distrito de Barranquilla, Colombia, durante los años 2014 a 2016. Metodología: estudio descriptivo, en el que se revisó de forma retrospectiva las historias clínicas de pacientes con patología mitral que requirieron intervención quirúrgica de tipo plastia, cambio biológico o mecánico, entre los años 2014 y 2016. El análisis de la información se realizó a través del software SPSS. Re­ sultados: durante el periodo de estudio se realizaron 276 cirugías valvulares en la institución de salud objeto de estudio, de las cuales 98 fueron cirugías sobre válvula mitral, excluyéndose 20 historias clí­ nicas que no contenían la totalidad de las variables analizadas. El antecedente clínico más frecuente fue la enfermedad coronaria (38,5%), seguido del infarto de miocardio (23,1%); el 20,5% de los pa­ cientes presentaron arritmia tipo fibrilación auricular y la cirugía sobre válvula mitral más realizada fue recambio biológico (42%). Conclusión: las características clínicas y quirúrgicas de los pacientes inclui­ dos en el estudio, son similares a las descritas en la literatura nacional e internacional, por lo que podría tomarse como precedente de presentación y de esta manera ir construyendo una clínica para la identificación rápida y veraz de los pacientes con alteraciones valvulares, permitiendo su abordaje temprano y disminuyendo la carga económica para el sistema de salud.

Introduction: cardiac valvular anomalies have increased their presentation, in part due to advances in diagnostic methods and knowledge of etiological predecessors as triggers of the clinical condition that initiates the diagnostic probability and early follow­up in patients in the initial stages of the di­ seas e, thus decreasing the economic impact due to increased morbidity and mortality. Objective: to describe the clinical and surgical characteristics of patients undergoing mitral valve surgery at a re­ference center in the district of Barranquilla during the years 2014 to 2016.Methodology: descriptive, retrospective, cross­sectional study in a health institution in the district of Barranquilla. Patients with mitral pathology who required surgical intervention of the plasty type, biological or mechanical chan­ ge, were included between 2014 and 2016. Data analysis performed in SPSS according to the natu­ re of the variables. Results : during the study period, 276 valve surgeries were performed, of which 98 were valve surgeries, 20 patients were excluded due to incomplete clinical history; the most pre­ valent antecedent was coronary disease in 38.5% followed by myocardial infarction with 23.1%; 20.5% of the patients presented arrhythmia type atrial fibrillation; the most performed mitral valve surgery was a 42% biological replacement. Conclusion: the clinical and surgical characteristics of the patients included in the study, correspond to those described in national and international litera­ ture, so it could be taken as a precedent for presentation and thus build a presentation clinic for quick and accurate identification of the patients with valvular alterations, allowing their early ap­ proach and decreasing the economic burden for the health system.

Comparison of PG-SGA, NRS 2002 and BIA in nutritional assessment and screening of patients with gynecologic cancers / 肠外与肠内营养

Objective:To evaluate the application of PG-SGA,NRS 2002 and BIA in nutritional assessment and screening of patients with gynecologic cancers.Methods:118 patients were randomly selected.Nutritional status were evaluated by PG-SGA,NRS 2002 and BIA,and consistency between each tools were compared.Results:The prevalence of malnutrition or nutritional risk of patients were 64.4％ (PG-SGA),57.6％ (NRS 2002),and 33.9％ (BIA) respectively.In all patients,the consistency of PG-SGA and NRS 2002 was high (P ＜ 0.001),while there were not significantly consistent between BIA and PG-SGA,or between BIA and NRS 2002 (P ＜ 0.001).Conclusion:According to the evaluation of PG-SGA or NRS 2002 in gynecologic patients,the prevalence of malnutrition or nutritional risk is high,and these two scales are suitable for nutritional assessment and screening of gynecologic cancer patients,especially in ovarian cancer patients.In addition,BIA may be a promising tool to evaluate cervical cancer patients' nutritional status.

Bioimpedance as a tool for evaluating the body composition of suruvi (Steindachneridion scriptum)

Abstract Bioelectrical impedance analysis (BIA) is regarded as an important tool for evaluating the body composition of different animals in a rapid, non-destructive, and low-cost manner. A South American fish species, Steindachneridion scriptum, known as suruvi, was selected for study in this investigation. A protocol to produce fish with different body composition was used to allow BIA to adequately predict the body composition of suruvi. The fish were fed twice each day with two different diets; a low lipid diet (8.90%), and a high lipid diet (18.68%). These dietary differences allowed suruvi specimens with different body compositions to be produced. The BIA readings were determined using a Quantum X Bioelectrical Body Composition Analyzer. Two readings (dorsal and ventral) were obtained for each fish. After BIA readings were obtained, the proximate composition of the fish bodies for each individual was determined. All of the study data were used to establish correlation equations between proximate analyses and BIA values. Strong correlations were found for S. scriptum. The highest correlations were obtained for the following pairs of quantities, using BIA data from dorsal readings: moisture and resistance in series (R2 = 0.87); protein and resistance in series (R2 = 0.87); and ash and reactance in parallel (R2 = 0.82). We conclude that BIA is an effective method in determining the body composition of S. scriptum without sacrificing the fish. However, to expand the use of this new technology it is important to define strict BIA protocols to guarantee accurate estimates.

Resumo A análise da impedância bioelétrica (BIA) é considerada uma importante ferramenta para avaliar a composição corporal de diferentes animais de uma maneira rápida, não-destrutiva e de baixo custo. A espécie Sul-americana Steindachneridion scriptum, popularmente conhecida como suruvi, foi selecionada para este estudo. Foi utilizado um protocolo para produzir peixes com distintas composições corporais, permitindo a validação da BIA para análise adequada da composição corporal do suruvi. Os peixes foram alimentados duas vezes ao dia com duas dietas diferentes: uma de baixo teor lipídico (8,90%) e outra de alto teor lipídico (18,68%). Essa diferença nas dietas possibilitou a produção de indivíduos com diferentes composições corporais. As leituras da BIA foram determinadas utilizando-se o equipamento Quantum X Bioelectrical Body Composition Analyzer. Duas leituras (dorsal e ventral) foram obtidas para cada peixe. Após as leituras, para cada peixe individualmente, a composição proximal dos peixes foi determinada. Todos os dados obtidos foram utilizados para estabelecer as equações de correlação entre as análises proximais e os valores da BIA. Fortes correlações foram encontradas para S. scriptum. As maiores correlações foram obtidas para as análises dorsais a seguir: umidade e resistência em série (R2 = 0,87); proteína e resistência em série (R2 = 0,87); cinzas e reactância em paralelo (R2 = 0,82). Pode-se concluir que o método BIA é eficiente em determinar a composição corporal do suruvi S. scriptum sem sacrificar o animal. No entanto, para expandir o uso desta nova tecnologia é necessário definir protocolos rigorosos para garantir estimativas precisas.

Bioimpedance as a tool for evaluating the body composition of suruvi (Steindachneridion scriptum) / Bioimpedância como ferramenta de análise da composição corporal do suruvi (Steindachneridion scriptum)

Abstract Bioelectrical impedance analysis (BIA) is regarded as an important tool for evaluating the body composition of different animals in a rapid, non-destructive, and low-cost manner. A South American fish species, Steindachneridion scriptum, known as suruvi, was selected for study in this investigation. A protocol to produce fish with different body composition was used to allow BIA to adequately predict the body composition of suruvi. The fish were fed twice each day with two different diets; a low lipid diet (8.90%), and a high lipid diet (18.68%). These dietary differences allowed suruvi specimens with different body compositions to be produced. The BIA readings were determined using a Quantum X Bioelectrical Body Composition Analyzer. Two readings (dorsal and ventral) were obtained for each fish. After BIA readings were obtained, the proximate composition of the fish bodies for each individual was determined. All of the study data were used to establish correlation equations between proximate analyses and BIA values. Strong correlations were found for S. scriptum. The highest correlations were obtained for the following pairs of quantities, using BIA data from dorsal readings: moisture and resistance in series (R2 = 0.87); protein and resistance in series (R2 = 0.87); and ash and reactance in parallel (R2 = 0.82). We conclude that BIA is an effective method in determining the body composition of S. scriptum without sacrificing the fish. However, to expand the use of this new technology it is important to define strict BIA protocols to guarantee accurate estimates.

Resumo A análise da impedância bioelétrica (BIA) é considerada uma importante ferramenta para avaliar a composição corporal de diferentes animais de uma maneira rápida, não-destrutiva e de baixo custo. A espécie Sul-americana Steindachneridion scriptum, popularmente conhecida como suruvi, foi selecionada para este estudo. Foi utilizado um protocolo para produzir peixes com distintas composições corporais, permitindo a validação da BIA para análise adequada da composição corporal do suruvi. Os peixes foram alimentados duas vezes ao dia com duas dietas diferentes: uma de baixo teor lipídico (8,90%) e outra de alto teor lipídico (18,68%). Essa diferença nas dietas possibilitou a produção de indivíduos com diferentes composições corporais. As leituras da BIA foram determinadas utilizando-se o equipamento Quantum X Bioelectrical Body Composition Analyzer. Duas leituras (dorsal e ventral) foram obtidas para cada peixe. Após as leituras, para cada peixe individualmente, a composição proximal dos peixes foi determinada. Todos os dados obtidos foram utilizados para estabelecer as equações de correlação entre as análises proximais e os valores da BIA. Fortes correlações foram encontradas para S. scriptum. As maiores correlações foram obtidas para as análises dorsais a seguir: umidade e resistência em série (R2 = 0,87); proteína e resistência em série (R2 = 0,87); cinzas e reactância em paralelo (R2 = 0,82). Pode-se concluir que o método BIA é eficiente em determinar a composição corporal do suruvi S. scriptum sem sacrificar o animal. No entanto, para expandir o uso desta nova tecnologia é necessário definir protocolos rigorosos para garantir estimativas precisas.

Comportamiento del virus de la bronquitis infecciosa aviar en aves con sintomatología respiratoria provenientes de granjas de producción del Departamento de Cundinamarca / Avian Infectious Bronchitis virus behavior in birds from commercial farms with a history of respiratory disease, located in the department of Cundinamarca

Objetivo. Evaluar la dinámica serológica contra el virus de bronquitis infecciosa aviar y su relación con la presentación y/o antecedentes de signos clínicos y hallazgos patológicos, bajo condiciones de campo. Materiales y métodos. Se realizó un muestreo al azar en dos fases, en pollo de engorde y reproductoras de granjas del Departamento de Cundinamarca. En la primera fase se tomó muestra de sangre a un total de 224 aves, distribuidas en 7 granjas. En la segunda fase, realizada 20 días posteriores al primer muestreo, se tomó una segunda muestra al mismo número de aves empleadas inicialmente. Las muestras de los sueros obtenidos se emplearon para la realización del inmunoensayo ligado a enzima (ELISA), diseñado para detectar anticuerpos frente al virus de la bronquitis infecciosa aviar en suero sanguíneo. Resultados. Se obtuvo que del total de las granjas analizadas el 85.72% mostró reactividad serológica al virus de la Bronquitis Infecciosa aviar (VBIA), con correlación ante la presencia de los signos clínicos o antecedentes respiratorios en granja.

Objective. Evaluate the serological dynamics against avian infectious bronchitis virus and its relationship with the presentation and / or a history of clinical signs and pathological findings, under field conditions. Materials and methods. A random sampling was conducted in two phases, in broiler and breeder farms located in the Department of Cundinamarca. In the first phase blood sample were taken from a total of 224 birds, distributed over the 7 farms. In the second phase, carried out 20 days after the first, a second sample was collected from the same number of birds used in the first phase. The serum samples were used to carry out the enzyme linked immunoassay (ELISA) intended to detect antibodies against avian infectious bronchitis virus in blood serum. Results. As a result it was found that from the total farms analyzed the 85.72% showed serologic reactivity against Avian Infectious Bronchitis Virus (AIBV) that correlated to the presence of clinical signs or previous history of respiratory disease in the farm.

A Novel, Potent, Small Molecule AKT Inhibitor Exhibits Efficacy against Lung Cancer Cells In Vitro / Journal of the Korean Cancer Association, 대한암학회지

PURPOSE: Anomalies of Akt regulation, including overexpression in lung cancer, impart resistance to conventional chemotherapy and radiation, thereby implicating this kinase as a therapeutic intervention point. A novel scaffold of Akt inhibitors was developed through virtual screening of chemical databases available at Birla Institute of Technology and Science, Pilani, Hyderabad, based on docking studies using Maestro. A benzothienopyrimidine derivative (BIA-6) was identified as a potential lead molecule that inhibited Akt1 enzyme activity with an IC50 of 256 nM. MATERIALS AND METHODS: BIA-6 was tested for in vitro Akt1 inhibition using a fluorescence resonance energy transfer kit. Anti-proliferative activity was tested in NCI-H460, A549, NCI-H1975, and NCI-H2170 cell lines. The effect of the compound on p-Akt (S473) was estimated. RESULTS: BIA-6 allosterically caused a dose dependent reduction of growth of cell lines with a half maximal growth inhibition (GI50) range of 0.49 muM to 6.6 muM. Cell cycle analysis indicated that BIA-6 caused a G1 phase arrest at < 100 nM but led to apoptosis at higher doses. BIA-6 also exhibited synergism with standard chemotherapeutic agents. CONCLUSION: BIA-6 is a novel, allosteric Akt inhibitor with potent anti-cancer activity in lung cancer cell lines, that effectively blocks the phosphoinositide-3 kinase/Akt pathway with a high margin selectivity towards normal cells.

INFLUENCE OF PERCENT BODY FAT WITH AND WITHOUT WATER IN BIOELECTRICAL IMPEDANCE ANALYSIS / 体力科学

Measuring the bioelectrical impedance (BI) is a simple and non-invasive method for estimating body fat or muscle mass. However, body impedance is affected by variations in the distribution of body fluid without reference to actual body fat or muscle mass. Twenty healthy college students (10 males, 10 females; mean age 21.0±2.3 years) participated in the study. Their mean body mass index was 20.7±2.6 kg/m². Bipolar electrodes were place on all extremities, and InBody 3.0^TM (Biospace Co., Ltd., Seoul, Korea) was used to measure bioelectrical impedance. Each subject remained in a supine position on a comfortable bed between 07:00 and 12:00 except for excretion and measurement of BI. BI was measured hourly using frequencies ranging from 5 to 500 kHz. The subjects refrained from eating, drinking and exercising between 07:00 and 12:00 during the first week of measurements, and drank 6.7 ml/kg of water at 07:00 after the first measurement of BI during a subsequent week of measurements. Bioelectrical impedance was higher in female subjects in all body segments and conditions (<i>p</i><0.01). BI in the right arm was lower than that in the left in all participants (<i>p</i><0.001). The difference between the highest and lowest BI among six measurements was largest in the upper extremities, followed by the lower extremities. Differences in the coefficient of variation CV values of the right arm of both females and males at 50, 250, and 500 kHz during fasting were significantly smaller than after drinking water. Hydration had no effect on the differences in the CV values of the body trunk and lower extremity BI or BI at lower frequencies. BI indicates the possibility of remarkable decrease in variation in the upper extremity BI at higher frequencies by taking 6.7 ml/kg of water at get up and enables minimizing the estimate error of body fat percentage.

Comparisons of obesity assessments in over-weight elementary students using anthropometry, BIA, CT and DEXA

Obesity was characterized in Korean elementary students using different obesity assessment tests on 103 overweight elementary students from three schools of Jeonbuk Province. The body mass index (BMI) and obesity index (OI) were compared, and the data using DEXA and CT were compared with the data using BIA and a tape measure. The results of this study are as follows: first, 27 students who were classified as obese by OI were classified as overweight by BMI, and 3 students who were classified as standard weight by BMI were classified as overweight by OI. Secondly, by DEXA and BIA measurements, there was 1.51% difference in body fat percentage (boys 1.66%, girls 1.17%) and the difference in body fat mass between boys and girls was 0.77 kg (boys 0.85 kg, girls 0.59 kg), but those differences in body fat percentage and mass were not statistically significant. Thirdly, the average total abdominal fat (TAF) measured by CT scans of obese children was more significantly related with subcutaneous fat (r = 0.983, P < 0.01) than visceral fat (r = 0.640, P < 0.01). Also, TAF were highest significant with waist circumference by a tape measure (r = 0.744, P < 0.01). In summary, as there are some differences of assessment results between two obesity test methods (BMI, OI), we need more definite standards to determine the degree of obesity. The BIA seems to be the most simple and effective way to measure body fat mass, whereas waist/hip ratio (WHR) using a tape measurer is considered to be the most effective method for assessing abdominal fat in elementary students.

Superiority for Neonatal Phenyiketenuria Screening by Chemical Fluorometric Enzyme Immunoassay / 医学研究杂志

0.05).Among 67 040 neonates screened by CFELA,11 1/6 096 were found to be defective;194 012 neonates screened by BIA,21 1/9 239 were found to be defective.Conclusion The guantitative CFELA is more sensitive and rapid,and should be a worthy method being recommend for major screening.

BODY COMPOSITION EVALUATED BY SEGMENTAL BIOELECTRICAL IMPEDANCE ANALYSIS IN HEALTHY SUBJECTS AND ATHLETES / 体力科学

The purpose of this study was to evaluate the fat-free mass (FFM) of the total body, the upper limbs, and the lower limbs in healthy subjects and basketball players obtained by the new 8-electrode segmental bioelectrical impedance analysis (S-BIA) and to compare the results with the FFM obtained by DXA. The participants were 30 healthy subjects (15 men and 15 women) aged 24.9±3.5 years old and 15 female basketball players aged 23.1±3.1 years old. In the healthy subjects, positive correlations (p<0.001) were observed between the FFM obtained by S-BIA and DXA of the total body (r=0.973), the upper limbs (r=0.956), and the lower limbs (r=0.954). Similarly, in the basketball players, positive correlations (p<0.001, p<0.01) were observed between the FFM obtained by S-BIA and DXA of the total body (r=0.943), the upper limbs (r=0.743), and the lower limbs (r=0.934). The results suggest that the new 8-electrode S-BIA is a valid and convenient method for analyzing body composition of the total body and the body segments in healthy subjects and athletes.

Validity of Bioelectrical Impedance Analysis (BIA) in Measurement of Human Body Composition / 소아과

PROPOSE: Body composition by bioelectrical impedance analysis (BIA) is a very useful method of analysing body composition. BIA is non-invasive, inexpensive, nonhazaedous and reproducible technique. The aim of this study was to determine the level of agreement between body composition measurement by BIA and dual-energy X-ray absorptiometry (DEXA). METHODS: Data was examined in 100 children (male 58; female 42), who visited Pusan National Hospital. Weight (kg) and height (cm) were measured, and body compositions were analyzed with fat mass, lean body mass, body fat percent by BIA and DEXA methods. RESULTS: Comparison of the DEXA and BIA methods showed highly statistically significant correlations in measurement of human body composition (fat mass, lean body mass, body fat percent). CONCLUSION: BIA should be considered as the method of choice in measurement of human body composition, since it's non-invasive, reliable, rapid, nonhazaedous and inexpensive, using portable equipment.

Ghrelin Levels During Puberty: Relationship with Anthropometric Measures and Other Hormones / 대한소아내분비학회지

PURPOSE: Puberty is a rapid changing periods of body composition and endocrine system. Already leptin was known to be closely related to body composition and was known to change according to Tanner stage. Ghrelin, a natural GH secretagogue, was also known to be related with weight change, fat utilization, insulin secretion, and influenced by sex steroid. But the relationship between ghrelin and other parameter according to pubertal development is not established yet. METHODS: Cross-sectional study of 101 peripubertal children (54 males and 47 females) aged 8.5 to 17.0 years. We examined body composition (free fat mass, fat mass, % body fat) by bioelectric impendence measurements. Total ghrelin, leptin, insulin was measured by radioimmunoassay (RIA). Relationship between ghrelin, leptin, insulin and body composition data was analysed according to Tanner stage controlling gender using SPSS ver 11.0. RESULTS: Ghrelin concentration is only correlated with Tanner stage (r=-0.21, P<0.05) and GIR (r=0.29, P<0.01). When we divided subjects by sex, even though there was no difference of ghrelin level between both sex, only male is correlated with Tanner stage (r=-0.28, P<0.05) and GIR (r=0.36, P<0.01). There was no difference of ghrelin level between each Tanner stage and no correlation was observed with other body composition and endocrine parameter including leptin. CONCLUSION: These data suggest that Ghrelin levels decrease during puberty at least in boys as pubertal development proceeds but it is minimal. During puberty, ghrelin levels are stable. Ghrelin may have no relationship with other pubertal change. The relation of ghrelin and insulin sensitivity need to be further investigated.

RELATIONSHIP BETWEEN NUTRITIONAL STATUS AND FLUID DISTRIBUTION IN CONTINUOUS AMBULATORY PERITONEAL DIALYSIS PATIENTS / 营养学报

Objective: To investigate the relationship between nutritional status and body fluid distribution in continuous ambulatory peritoneal dialysis (CAPD) patients. Methods: Sixty-six CAPD patients without edema were recruited in this study. Patients’ fluid status was measured by bioimpedance analysis (BIA) and nutritional status was assessed by subjective global assessment (SGA). Thirty-six age-matched healthy people served as controls. Results: As compared to the healthy control, CAPD patients had significant abnormal fluid distribution in the body with significantly decreased intracellular water (ICW) and little change in extracellular water (ECW). Malnutrition further aggregated the changes in body fluid distribution. Conclusion: Body fluid distribution is abnormal in CAPD patients and may be affected by patients’ nutritional status. The ratio of ICW/ECW can not be correctly used to evaluate hydration state when the patient is malnourished. Key word: intracellular water (ICW); extracellular water (ECW); bioimpedance analysis (BIA); malnutrition

Association of Anthropometric Indices with Prevalence of Hypertension in Korean Adults / 예방의학회지

OBJECTIVE: To examine the relationship between hypertension prevalence and the four commonest anthropometric measurements for obesity(body mass index(BMI), wasit-hip ratio(WHR), waist circumference(WC) and body fat in Korean adults. METHODS: We studied the cross-sectional association of the anthropometric indices and blood pressure in 1,197 individuals( who were participants in the population-based cohort study). Hypertension was defined as blood pressure 160/95 mmHg or current use of antihypertensive medication. Informations on life-style factors were obtained from personal interview. RESULTS: There were close associations between BMI, WHR and WC with blood pressure in both men and women. After age adjustment, BMI and WC showed significantly positive correlation with systolic and diastolic blood pressure levels in both men and women. Odds ratio(ORs) of being hypertensive were estimated comparing the highest to the lowest quantile, adjusting for age, smoking status, alcohol intake levels, education attainment. The simultaneously adjusted ORs of being hypertensive, comparing the highest vs the lowest categories, was for BMI 2.0(95% confidence interval(CI)=0.9-3.2) in men and 3.2 (95% CI=1.7-6.1) in women, for WC 2.1(95% CI=1.0-4.4) in men and 3.1(95% CI=1.6-5.9) in women, for fat(%) 4.2(95% CI=1.9-9.5) in men and 2.1(95% CI=1.2-3.6) in women. CONCLUSION: In addition to measures of overall obesity(BMI) as well as central obesity(WHR, WC), body fat(%) was independently associated with prevalence of hypertension. Among obesity indices, body fat was the most predictor variable in hypertensive state in male and BMI was in female.

The Availability of Bioelectrical Impedance Analysis for Estimating the Obesity on the Relative Risk in the Breast Cancer / 한국유방암학회지

BACKGROUND: The incidence of female breast cancer has been increasing during last 10 years. There are many risk factors in breast cancer. We evaluated the relative risk of breast cancer in relation to obesity and body composition. Also we evaluated the Bioelectrical Impedance Analysis (BIA) for estimating the obesity. MATERIALS AN METHODS: The data were collected as a case-control study from September 1, 1997 to August 31, 1998. Cases were selected from newly diagnosed and treated breast cancer patients at Breast Clinic of Gil Medical Center, Gachon Medical College. Control group was randomly selected form women with benign breast disease or women in screening rest. All suspected risk factors including obesity indices and body composition were checked. Especially body fat percent, total body water and lean body mass were checked by BIA. RESULTS: There was no association between the relative risk of breast cancer and education, smoking, drinking, oral contraceptives use, reproductive factors and breast feeding factors. Data analysis showed the high possibility of positive relationship between early menarche factors( or =51 years old ) and the brest cancer, however there was no statistical significance. There was a statistical significance between risk group and control group in fat percent (p=0.0187) and WHR (waist-hip ratio) (p=0.0174) among obesity indicies. BMI & obesity (In the classification if Obesity by Japan Society for Study of Obesity) were associated with relative risk of breast cancer by menopausal status. In premenopausal women, breast cancer risk decreased in high BMI & obese group, however the increase was shown in postmenopausal women contrarily. . CONCLUSIONS: This study suggests that there I a significant difference in the relationship between the obesity index and the relative risk of breast cancer between Korean and Western women. Bioelectrical Impedance Analysis is a available method for estimating of obesity in relative risk of breast cancer women. Further studies will be needed for evaluating the role of BIA and the relationship of obesity with staging & prognosis of breast cancer.

Bioelectrical Impedance Analysis (BIA) of the Estimation of Total Body Water and Lean Body Mass in Patients with Renal Failure / 대한신장학회잡지

Bioelectrical impedance analysis (BIA) was used to determine total body water (TBW) and lean body mass (LBM) in patients with renal failure. The body's electrical resistance (R) was measured by the voltage to current ratio, injecting an 800microA alternating current with a frequency of 50KHZ and detecting a voltage drop between the wrist and the ankle. Impedance index (Height2/Resistance) compared favorably with TBW measured by deuterium (D2O) dilution method as the reference, giving the correlation coefficient (r) of 0.966 and standard error estimation (SEE) of 2.71 liter. The index was compared with LBM determined by dual-energy x-ray absorptiometry (DEXA) as the reference, giving r of 0.970 and SEE of 3.00kg. The r of 0.985 and SEE of 2.15kg were found between the reference method. BIA appeared to have a somewhat lower accuracy than those of the reference method. However, it is a useful clinical tool for estimating body composition, because it is easy, rapid and non-invasive. The existing BIA method is based on an extremely simple conductor model of the body. The accuracy may be improved further, based on a more realistic model for the body.

Relationship between Lean Body Mass(LBM) by Bioelectrical Impedance Analysis(BIA) and Nutritional Parameters in Hemodialysis Patients / 대한신장학회잡지

The incidence of protein and energy malnutrition is about 20-40% in patients on maintenance dialysis and it is an important determinant of morbidity and mortality in end-stage renal disease. The traditional methods of nutritional assessment such as body measurement and dietary assessment are relatively ineffective because of operator dependency. Biochemical data, also, have the difficulty in early detection of malnutrition. Therefore more precise and simple method such as bioelectrical impedance analysis(BIA) was needed. We perfomed a study of 25 maintenance hemodialysis patients who were clinically stable and had been on dialysis over 3 months to identify the relationship between lean body mass(LBM) by BIA and other nutritional indicators. Mean age was 43+/-12 years, sex ratio, M:F=1:1.3, and mean dialysis duration, 56+/-38.9 month. Three patients were diabetes mellitus(12%), Delivered Kt/ Vurea, serum albumin, and %LBM were 1.5+/-0.2/ session, 4.1+/-0.4g/dl and 76.5 +/-10.5%, respectively. Patients were divided into 2 groups; group I whose %LBM was less than 75%, and group II whose %LBM was more than 75%. Patients were significantly older in group I, but sex ratio, percentage of diabetes mellitus, dialysis duration were not significantly different between two groups. Serum cholesterol(175.3+/-35.2 vs. 142.5+/-24.7mg/dL) was significantly higher and serum creatinine(12.4+/-2.7 vs. 16.2+/-4.3mg/dL) was significantly lower in group I. Serum albumin and insulin-like growth factor-I (IGF-I) were similar between two groups. On data measured by BIA, LBM(67.9+/-6.1 vs. 84.4+/-1.9%), height2/resistance(39.4+/-4.3 vs. 52.7+/-8.5) were significantly lower in group I. %Body fat was significantly higher in group I(31.3+/-4.6 vs. 15.7+/-6.7%). Dietary protein intake(30+/-13.5 vs. 41.7+/-14.6g/day) and total calorie intake(1239.9+/-272.3 vs. 1329.4+/-375.0 Cal/day) were higher in group II. %LBM correlates positively with serum creatinine (r=0.41, p<0.05) and dietary protein intake(r=0.51, p<0.05), but negatively with age(r=-0.59, p<0.05) and cholesterol(r=-0.52, p<0.05). In conclusion, %LBM by BIA in maintenance hemodialysis patients appears to be well correlated with traditional nutritional study.

The screening of C5a antagonist by optic biosensor technique / 中国免疫学杂志

Objective:To screen the antagonist of C5a anaphylatoxin,and further to study the dinetic characteristics of interaction between C5a and its anti-sense peptides.Methods:The anti-sense peptides were screened that can interact with C5a selectively by means of dinetic analysis,via biosensor technique.Results:There is one piece of anti-sense peptide(R4) being screened from these four synthesized peptides.The dissociation equilibrium constant(K D) between R4 and the immobilized hC5a is 6.62?10 -6 mol/L and the K D between R4 and L2 is 7.02?10 -7 . Conclusion:Based on the results obtained,it was concluded that the optic biosensor is a ideal and powerful tool to facilitate the kinetic analysis of interaction between sense peptide and its anti-sense peptide and to screen antagonist of biologic activity molecule.